悬钩子属种质的评价

在进行了７省悬钩子资源调查的基础上,在南京建立了悬钩子属田间基因库。３年来对田间基因库内保存并开花结果的３０个种进行了开花结果性状的记栽和评价。内容包括果实特征,糖、酸、维生素类、氨基酸、矿质元素含量分析,染色体计数等。分析了种间和种内多样性物存在及在良种选育中利用的可能性。     

人乳腺癌cAMP结合蛋白

建立了测定人乳腺癌胞浆ｃＡＭＰ结合蛋白（ｃＡＭＰｂ．ｐ．）方法。综合研究了其温度、保温时间、配体浓度、稳定性等条件。ｃＡＭＰｂ．ｐ．的Ｋ_Ｄ值为２．９０×１０~（－８）ｍｏｌ／Ｌ．并测定了６０例雌激素受体（ＥＲ）Ｆｕ性乳腺癌标本的ｃＡＭＰｂ．ｐ．含量。此组病人术后均接受系统的内分泌治疗,ＥＲ／ｃＡＭＰｂｐ,比值范围为７．７～３６２×１０~（－３）,ＥＲ／ｃＡＭＰｂ．ｐ．比值≥４０×１０~（－３）的五年生存率明显高于比值＜４０×１０~（－３）组,（ｐ＜０．００５）．表明测定ＥＲ／ｃＡＭＰｂ．ｐ．比值对预测患者内分泌治疗疗效,优于单独测定ＥＲ．     

Characterization of a Chlamydomonas reinhardtii gene encoding a protein of the DNA photolyase/blue light photoreceptor family

The organization and nucleotide sequence of a gene from Chlamydomonas reinhardtii encoding a member of the DNA photolyase/blue light photoreceptor protein family is reported. A region of over 7 kb encompassing the gene was sequenced. Northern analysis detected a single 4.2 kb mRNA. The gene consists of eight exons and seven introns, and encodes a predicted protein of 867 amino acids. The first 500 amino acids exhibit significant homology with previously sequenced DNA photolyases, showing the closest relationship to mustard (Sinapis alba) photolyase (43% identity). An even higher identity, 49%, is obtained when the Chlamydomonas gene product is compared to the putative blue-light photoreceptor (HY4) from Arabidopsis thaliana. Both the Chlamydomonas and the Arabidopsis proteins differ from the well characterized DNA photolyases in that they contain a carboxyl terminal extension of 367 and 181 amino acids, respectively. However, there is very little homology between the carboxyl terminal domains of the two proteins. A previously isolated Chlamydomonas mutant, phrl, which is deficient in DNA photolyase activity, especially in the nucleus, was shown by RFLP analysis not to be linked to the gene we have isolated. We propose this gene encodes a candidate Chlamydomonas blue light photoreceptor.     

不同时期羊草群落光合速率与环境条件之间的关系模型

利用１９８１－１９８５年间,内蒙古典型草原羊草群落光合速率和环境因子观测资料,按４个不同时期进行逐步多元回归,得到４个回归方程如下：１．６月上中旬植物生长初期,群落光合主要与光照强度和温度有关。其回归方程是ｙ＝－０．６８３＋０．４４４ｘ１－０．０３９１ｘ＋０．０７４２ｘ３－０．００１６４ｘ＾２３（式中ｙ为羊草群我合速率ｇＣｏ２／ｍ＾７２．ｈ,ｘ１是光照强度１０ｋｌｘ,ｘ３为空气温度Ｃ）。方程的复相     

橡胶-砂仁复合系统生物产量、营养元素空间格局的研究

通过对橡胶与砂仁间作林和同龄纯胶林各层次各器官生物量及１０种营养元素含量的测定,对比研究了两种分乔木层生物量按径级分配,林分平均净生产量,营养元素的积累分布规律,林龄为３０ａ,间作龄为８ａ的胶砂间作林的总生物量为１３９．８５ｔ／ｈｍ＾２。其中乔木层占７２．７％,间作层占２２．６％,枯落物层占５．２％,与同龄纯胶林相比,乔木层提高了７．６％,枯落物层提高了３８．９％,两种林分乔木层地上部分生物量约占     

Cloning of cDNA for Vitellogenin of the Parasitoid Wasp, Pimpla nipponica (Hymenoptera: Apocrita: Ichneumonidae): Vitellogenin Primary Structure and Evolutionary Considerations

The cDNA for vitellogenin (Vg) of the parasitoid wasp Pimpla nipponica (Hymenoptera: Apocrita) was cloned and sequenced.¹ The deduced amino acid sequence with 1807 residues was obtained. The N-terminal 20 amino acids chemically determined for vitellin (Vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. The cDNA clone for the Vg of the turnip sawfly Athalia rosae (Hymenoptera: Symphyta), previously obtained and partially sequenced, was also completely sequenced and the amino acid sequence deduced. Amino acid sequences were compared between these two species and also with known Vg sequences from other insects. Common to all these insects is the presence of two long regions with relatively well-conserved amino acid sequences, one near the N-terminal extending 267–282 residues (including two cysteines at conserved locations), and the other starting at position 450 to 655 and extending 279–283 residues, and of a region at the C-terminal extending some 200 residues (about 250 in Aedes aegypti due to the presence of a serine-rich stretch) with 10 cysteines at conserved locations. A molecular phylogenetic tree was constructed.     

四种鱼类外周血红细胞细胞周期及DNA含量

通过流式细胞仪测量了兴国红鲤（Ｃｙｐｒｉｎｕｓ ｃａｒｐｉｏ Ｖａｒ． Ｓｉｎｇｕｏｎｅｎｓｉｓ）、奥利亚罗非鱼（Ｓａｒｏｔｈｅｒｏｄｏｎ ａｕｒｅａ）、 鳙（Ａｒｉｓｔｉｃｈｔｈｙｓ ｎｏｂｉｌｉｓ Ｒ．）和团头鲂（Ｍｅｇａｌｏｂｒａｍａ ａｍｂｌｙｃｅｐｈａｌａ Ｙｉｎ．）红细胞的ＤＮＡ含量,四种鱼与鸡红细胞ＤＮＡ含量的比值分别为１·６５、０．９６、０．９１、１·１５,以鸡红细胞ＤＮＡ含量２．３ｐｇ／Ｎ计算,四种鱼二倍体体细胞的 ＤＮＡ含量分别为３．８０ ｐｇ／Ｎ, ２． ２２ｐｇ／Ｎ、２． ０８ｐｇ／Ｎ、 ２． ６６ｐｇ／Ｎ。另外,四种鱼外周血红细胞分别有２６％、 ２６％、 ２３％、 ２４％的细胞处于 ＤＮＡ合成期（Ｓ）,ＤＮＡ合成后期（Ｇ２）和分裂期（Ｍ）,这表明这四种鱼类的外周血红细胞并非失去分裂能力的特化细胞群,它们表现了较强的细胞周期现象。这有可能是这几种硬骨鱼类外周血仍具有造血功能的缘故。     

哺乳动物乳蛋白基因的表达与调控

本文在介绍了乳蛋白基因组结构及进化关系的基础上,就其乳蛋白基因表达的影响因素：顺式调控成分,反式作用因子及激素对表达的诱导进行了讨论,最后就该系统作为生物反应器的开发前景作了展望。     

脑内微透析采样技术及其在神经科学中的应用

作为一种新的在体化学采样技术,脑内微透析引起了神经科学家的关注。它与迅速发展起来的高灵敏度的微量分析技术相结合,实现了对体内细胞外环境中化学物质变化的动态监测,从而在神经科学领域获得应用。本系统地介绍了这一新技术的原理和方法,并扼要地介绍了一这一技术在神经科学中的应用及其取得的新进展,并结合本实验室的工作经验,对该技术存在的一些问题进行了讨论。     

高必需氨基酸转基因马铃薯的研究

８０年代以来,马铃薯遗传转化系统日趋成熟,转基因工程植株已被广泛应用于基础科学研究［１］。作为食物蛋白和能量主要来源的马铃薯,提高其蛋白质含量及质量的遗传工程研究正受到人们的普遍关注［２］。Ｙａｎｇ等［２］将旨在改善氨基酸平衡的ＣＡＴ－ＨＥＡＡＥ（氯酶素乙酰转移酶－高含量人体必需氨基酸）融合基因导入马铃薯,获得了Ｓｏｕｔｈｅｒｎｂｌｏｔ、Ｎｏｒｔｈｅｒｎｂｌｏｔ、Ｗｅｓｔｅｒｎｂｌｏｔ的证据,但尚缺少氨基酸分析的资料。玉米醇溶蛋白（ｚｅｉｎ）［３］是一个富含甲硫氨酸的贮存蛋白,它和人工合成的ＨＥ…